Topic: sample-preparation

What is depth of field and how can I optimize it in a scanning electron microscope?

By Luigi Raspolini - May 3, 2018

Imaging with a scanning electron microscope (SEM) consists of taking pictures of small features. So why not consider a comparison with photography? Let’s analyze how similar the behaviors of a SEM and a camera are when it comes to focusing on your subject, and what the exact definition of depth of field is.

Tip: Get a free demo of our Desktop SEM and discover it's capabilities for your research
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Imaging with a scanning electron microscope (SEM) consists of taking pictures of small features. So why not consider a comparison with photography? Let’s analyze how similar the behaviors of a SEM and a camera are when it comes to focusing on your subject, and what the exact definition of depth of field is.

Tip: Get a free demo of our Desktop SEM and discover it's capabilities for your research
Request your personal demo

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How SEM helps research polymers characteristics, properties, and uses

By Luigi Raspolini - Apr 19, 2018

Polymers have many uses and applications: engineered combinations of monomers produce a nearly infinite number of molecules with different properties, which are determined by the chemical composition and structure of the molecule. The form of the molecule has a big influence on how the polymer will behave when exposed to different external forces. In this blog, you’ll find practical examples of how Scanning Electron Microscopes (SEMs) can provide unexpected results.

Polymers have many uses and applications: engineered combinations of monomers produce a nearly infinite number of molecules with different properties, which are determined by the chemical composition and structure of the molecule. The form of the molecule has a big influence on how the polymer will behave when exposed to different external forces. In this blog, you’ll find practical examples of how Scanning Electron Microscopes (SEMs) can provide unexpected results.

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How-to: high-quality fiber analysis through proper SEM sample preparation

By Karl Kersten - Jan 18, 2018

Fibers are generally imaged in a scanning electron microscope (SEM), which provides high-resolution images, elemental analysis, and the possibility of automatically measuring thousands of fibers in mere minutes. But in some cases, imaging fibers with a SEM also presents challenges as the nature of some fibers might compromise the quality of your analysis. With this in mind, this blog describes how you can obtain high-quality imaging and fiber analysis through proper SEM configuration and sample preparation.

Fibers are generally imaged in a scanning electron microscope (SEM), which provides high-resolution images, elemental analysis, and the possibility of automatically measuring thousands of fibers in mere minutes. But in some cases, imaging fibers with a SEM also presents challenges as the nature of some fibers might compromise the quality of your analysis. With this in mind, this blog describes how you can obtain high-quality imaging and fiber analysis through proper SEM configuration and sample preparation.

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Sample preparation techniques for SEM to neutralize the effect of vacuum

By Luigi Raspolini - Aug 24, 2017

Scanning electron microscopes (SEM) scan the surface of the sample with an electron beam, collecting reflected electrons which carry information about the material the electrons interact with. If gas is in the sample chamber, its atoms interact with the beam, partly deflecting electrons and adding noise to the image. 

This is the reason why vacuum must be achieved in SEM before imaging. But while vacuum is crucial for proper analysis, it can also have a negative effect on certain types of materials. Read this blog to learn how you can neutralize vacuum and keep your samples intact.

Scanning electron microscopes (SEM) scan the surface of the sample with an electron beam, collecting reflected electrons which carry information about the material the electrons interact with. If gas is in the sample chamber, its atoms interact with the beam, partly deflecting electrons and adding noise to the image. 

This is the reason why vacuum must be achieved in SEM before imaging. But while vacuum is crucial for proper analysis, it can also have a negative effect on certain types of materials. Read this blog to learn how you can neutralize vacuum and keep your samples intact.

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Expert sample preparation techniques for SEM

By Luigi Raspolini - Aug 17, 2017

When using a scanning electron microscope (SEM) for the first time, you might have doubts about what can be imaged. You might also struggle to get the image quality you were expecting. Luckily, you can easily improve your results by following the simple yet powerful sample preparation techniques for SEM in this blog. Read on! 

When using a scanning electron microscope (SEM) for the first time, you might have doubts about what can be imaged. You might also struggle to get the image quality you were expecting. Luckily, you can easily improve your results by following the simple yet powerful sample preparation techniques for SEM in this blog. Read on! 

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SEM sample tilting: how to keep the area of interest within the field of view

By Luigi Raspolini - Aug 10, 2017

Certain samples are tricky to image. Sometimes, even the best sample preparation will be of no help in finding the results you need. Surface roughness and features on top of the sample might hide the specific area of interest, which could contain crucial information about surface defects or characteristics of the imaged material. In cases like this, you need a new point of view. Read this blog to discover how you can get just that.

Certain samples are tricky to image. Sometimes, even the best sample preparation will be of no help in finding the results you need. Surface roughness and features on top of the sample might hide the specific area of interest, which could contain crucial information about surface defects or characteristics of the imaged material. In cases like this, you need a new point of view. Read this blog to discover how you can get just that.

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